Which I pray before thee night and day He refers to all the prayers which he had for some time been addressing to God, during his sorrow for the desolations of Jerusalem.
Related Commentaries of Nehemiah 1:6
Nehemiah 1:6
6 Let thine ear now be attentive, and thine eyes open, that thou mayest hear the prayer of thy servant, which I pray before thee now, day and night, for the children of Israel thy servants, and confess the sins of the children of Israel, which we have sinned against thee: both I and my father's house have sinned.