Romans 16:12 - Albert Barnes' Notes on the Bible

Tryphena and Tryphosa - These names, with the participle rendered “who labor,” are in the feminine gender, and these were probably two holy women, who performed the function of deaconesses, or who ministered to the sick, and who with Persia, thus by example, and perhaps by instruction, labored to promote the spread of Christianity. Pious females, then, as now, were able to do much in their proper sphere to extend the truths and blessings of the gospel.