“A little while and you see me no more, and again a little while and you will see me.”
The meaning of this verse is amplified in the following verses. He is departing from them and in human terms they will not see Him again after the following day. But shortly afterwards they will see Him for He will rise again and they will see Him face to face as the glorified Christ, and from then on the Spirit will reveal Him continually to them as such. That ‘you will see me' does not refer to the second coming is apparent in John 16:20-24.