He repeats his former question, Genesis 43:27, either because he questioned the truth of their former relation, or would be further satisfied in it, it being usual with men to ask over and over again what they long to know; or because he now desired a more particular relation of his father's condition, and how he did bear up under all his calamities. They were troubled at his presence, from a sudden and deep sense of their horrid guilt, and their just fear of some dreadful punishment.
Related Commentaries of Genesis 45:3
Genesis 45:3
3 And Joseph said unto his brethren, I am Joseph; doth my father yet live? And his brethren could not answer him; for they were troubledb at his presence.